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The major semaglutide API manufacturers globally include Novo Nordisk A/S as the originator with the largest captive production capacity at its Kalundborg, Denmark facility

However, it creates a regulatory exemption (under the FD&C Act) that allows compounders to manufacture the drug without an NDA

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DNA methylation Genomic DNA was isolated from the liver tissue or cultured cells using an AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Germantown, MD) following the manufacturers instructions